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For ferri- and ferromagnetic substances, the magnetic mass susceptibility is dependent upon the magnetic field strength (H), as the magnetization of such substances is not a linear function of H but approaches a saturation value with increasing field
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Subcellular fractionation assay Freshly harvested brain cortex, hippocampus, or cells were washed once with ice-cold PBS, suspended in isolation buffer, consisting of 250 mmol/L sucrose, 10 mmol/L KCl, 1.5 mmol/L MgCl 2 , 1 mmol/L Na 2 EDTA, 1 mmol/L DTT, 1 mmol/L PMSF, 10 mmol/L Tris-HCl (pH 7.4), and protease inhibitor cocktail, and incubated on ice for 30 minutes, followed by homogenization